GIEMSA solution

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Giemsa solution is used for differentiation of nuclear and/or cytoplasmatic morphology of lymphocytes, monocytes, granulocytes (neutrophils, eosinophils, basophils), thrombocytes and erythrocytes. There are various methods of using the Giemsa solution, and the so-called Pappenheim method is one of the most commonly used ones. The method is esentially the May-Gruenwald Giemsa method combined with the May-Gruenwald solution that stains cytological material (peripheral blood smears, cytodiagnostic puncture aspirates, diarrhea or secretion cells) or hematopoietic organs’ sections. Along with the Pappenheim method, the Giemsa solution is commonly used for chromosomatic aberations detection in cytogenetics.

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